Production and characterization of monoclonal antibodies against chicken lymphocyte surface antigens.
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چکیده
منابع مشابه
Production and Characterization of Monoclonal Antibodies against Brucella abortus S (99) Surface Antigens
By immunizing mice with killed whole bacterial cells of Brucella abortus S (99), a panel of six hybridomas producing monoclonal antibodies (mAb) specific for the surface antigens of this bacterium were produced. ELISA was used to screen the hybridoma supernatants. Immunoblots of the cell extract indicated that three mAb were specific for S-LPS (Ba-1, Ba-2, Ba-3) and three others were reactive w...
متن کاملproduction and characterization of monoclonal antibodies against brucella abortus s (99) surface antigens
by immunizing mice with killed whole bacterial cells of brucella abortus s (99), a panel of six hybridomas producing monoclonal antibodies (mab) specific for the surface antigens of this bacterium were produced. elisa was used to screen the hybridoma supernatants. immunoblots of the cell extract indicated that three mab were specific for s-lps (ba-1, ba-2, ba-3) and three others were reactive w...
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Monoclonal antibodies (Mabs) were produced against chicken IgY purified from egg yolks. Purity of the IgY was established using immunoelectrophoresis and reducing and non-reducing polyacrylamide gel electrophoresis. Five stable hybrid clones were characterized and Mabs, all belonging to IgG1 isotype, were found to bind to the IgY heavy chain in Western blots. These clones did not cross-react wi...
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The production of murine monoclonal antibody (MAb) has not yet been reported in Iran. The present work describes for the first time the generation of several murine hybridoma clones secreting MAbs directed against human leukocyte surface antigens. The secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. Results indicated that of seven h...
متن کاملProduction and Partial Characterization of Monoclonal Antibodies to Leishmania
Background: Monoclonal antibody technology allows identification of amastigote-specific antigen in human tissue biopsies and search for a suitable vaccine candidate, which may induce a long lasting immunity. Objectives: Production and partial characterization of seven monoclonal antibodies against Leishmania tropica promstigotes. Methods: Seven murine monoclonal antibodies of the IgG isotype we...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1990
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.52.97